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When the OD 600 reached 0.60.8, the culture medium was placed in ice water for 30 min and was induced by adding 0.25 mM isopropyl--D-thiogalactopyranoside (IPTG) at 18 C with shaking at 100 rpm for 20 h

"hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates GATA-mediated activation"

doi: 10.1016/j.biopsych.2009.05.027 108 JamesSJShpylevaSMelnykSPavlivOPogribnyIP

10.1038/s41419-023-05835-8

[DOI] [PMC free article] [PubMed] [Google Scholar] Gladstone DJ, Black SE, Hakim AM
