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Within the brain, GSH abundance appears to vary by cell type (Huang & Philbert, 1995

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Metabolite profiles in the anterior cingulate cortex of depressed patients differentiate those taking N-acetyl-cysteine versus placebo

After treatments, the slides were fixed with 4% paraformaldehyde at 37C for 30 min, permeabilized with 0.2% Triton X-100 (Beyotime Institute of Biotechnology) at 37C for 20 min, blocked with 5% bovine serum albumin (BSA, Beijing Solarbio Science & Technology Co., Ltd.) at 37C for 30 min and incubated with primary antibodies against HMGB1 (1:100) and GPX4 (1:100) at 4C overnight

[DOI] [PubMed] [Google Scholar] [5].Wilchek M, Affinity chromatography, Die Angew