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Here is the daily recommended allowance of vitamin B12 for different age groups of individuals:

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doi: 10.1038/nature09782 16 SahaSPanigrahiDPPatilSBhutiaSK

Upon arrival at UTMB, the 18-week-old mice were single-housed and provided ad libitum access to water and a Western diet (WD
MTT (5 mg) was placed in a 5 mL EP tube, and PBS buffer (1 mL) added to dissolve the MTT