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Furthermore, although the two domains of the Fv fragment, V L and V H , can be coded for by separate genes, they can be joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the V L and V- regions pair to form monovalent molecules (known as single chain Fv (scFv)

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It is the most common route for musculoskeletal applications because it allows proximal injection near the injury site, which appears to improve local tissue concentration based on animal study findings

Apply within 30 minutes after training to maximize anabolic response

Apply to damp face, distribute with circular massaging movements