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Tissue was centrifuged at 200 g to remove the trypsin buffer and replaced with IBM1 buffer containing 50 mM KCl, 67 mM sucrose, 50 mM Tris pH7.4, 10 mM EDTA, and 0.2% BSA before homogenization via mechanical shearing using a glass Dounce homogenizer

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Luh et al, 2017) was used to assess motor coordination

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