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Description
The p38 MAPK (also called PMK-1) pathway is activated through phosphorylation by upstream MAPKK (SEK-1), which is activated by MAPKKK (NSY-1)

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Economic penalty extends beyond downtime: flow-agent addition (1-2% silica) adds $0.15-$0.30/kg to bill-of-materials, while rejection rates above 3% further erode gross margin

Reproduced with permission from WHO Classification of Tumours Editorial Board

C-terminally 6xHis-tagged HID was amplified using RF1396 and RF1397 and cloned into pACYC-Duet (MCS1) linearised using RF1398 and RF1400 using Gibson assembly, and SLP L or LID were amplified using RF1394 and RF1395 or RF1395 and RF1396, respectively, and cloned into MCS2 using Nde I- Kpn I restriction cloning
