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Expression and purification of Authentic M pro and C300S M pro The SARS-CoV2 M pro -encoding sequence and C300S mutant sequence were cloned into pGEX-4T1 vector (Genscript) with N-terminal self-cleavage site (SAVLQ/SGFRK) and C-terminal His 6 -tag as previously designed by others 6
Kosfeld M, Heinrichs M, Zak PJ, Fischbacher U, Fehr E
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In a study by Zhu et al., it was reported that the heptamethine dye dc-IR825 can trigger excessive mitophagy in A549 cells 136
