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RNA purification from 50 mg of root tissues was performed according to the manufacturers instructions using Quick-RNA Miniprep Kit (Zymo Research)

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PLoS Pathog 2:e27 Lynn GE, Cerny J, Kurokawa C, Diktas H, Matias J, Sajid A, Arora G, De-Ponte K, Narasimhan S, Fikrig E (2022) Immunization of guinea pigs with cement extract induces resistance against Ixodes scapularis ticks