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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

89 observed that the significant elevation in EL levels following Pb application underscores the disruption of membrane integrity and highlights the oxidative stress induced by Pb in barley plants under Pb stress conditions

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It provides the best balance of stability, convenience, and cost for standard cell culture and biochemical assays

Peptides play a crucial role in various physiological processes, including hormone regulation, immune function, and cellular repair