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After euthanasia, the pancreas was perfused via the common bile duct with 24 mL of digestion buffer (Hanks Balanced Salt Solution containing type IV collagenase, 1 mg/mL, Gibco, 17104019)

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Pharmacol Rep 72:769782 Carnero A, Blanco-Aparicio C, Renner O, Link W, Leal JF (2008) The PTEN/PI3K/AKT signalling pathway in cancer, therapeutic implications