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10.1073/pnas.94.11.5594 Proc Natl Acad Sci USA
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Briefly, 100 ng recombinant PKC was incubated with 40 M ATP, 40 mM MgCl2, 2 mM CaCl2, 60 g/mL phosphatidylserine (PS), 2 g/mL dioleoylglycerol (DG) in Tris-HCl 20 mM pH 7.4 at 37 C for 30 minutes
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