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Accessed 2/25/2015

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Then, the gel pieces were removed by centrifugation, and the protein-containing supernatants were collected and washed 3 times by Amicon Ultra-0.5 Centrifugal Filter Unit (3 kDa MWCO), to remove salts and impurities diffusing from the gel during the extraction

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To assess whether pharmacological inhibition of Ire1 can also alleviate IRI, we treated wild-type C57BL6 mice with vehicle, 48c, or liproxstatin-1 (a ferroptosis inhibitor used as a positive control for reducing IRI Ref