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Additionally, hIDO1 has a broad substrate specificity, catalyzing the oxygenation of a variety of indoleamines such as 5-hydroxytryptophan, 1-methyltryptophan, 5-methyltryptophan and 5-fluorotryptophan [135] and even serotonin in other organisms [136]

In short, RBCs were left untreated or exposed to an isotonic AAPH-containing solution for 1 h at 37 C with or without pre- or post incubation with the anthocyanin extract (0.01 g/mL) for 1 h at 37 C)

The key comparison was between the KC group (KD with biotin) and the KBD group (KD without biotin), which allowed the researchers to assess whether KD alone increases biotin requirements or accelerates the development of biotin deficiency