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The ribonucleoprotein (RNP)-mediated method was applied by constructing a complex containing the crRNA (5-CCACCTGTTGGTCGCACTCGGGG-3), tracrRNA-ATTO550, and the spCas9 protein (IDT Technologies)
A 500mcg daily protocol under split dosing would look like: 300mcg upon waking (fasted), then 200mcg approximately 30 minutes before dinner or before bed on an empty stomach
10.22038/ajp.2016.6187 197 RaiB.KaurJ.JacobsR.SinghJ
however, the control cells did not [37]
