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The colonic muscle is the most oxygenated region with 7%10% oxygen levels (Schwerdtfeger et al., 2019)

Statements Author contributions DY, FC, and XL contributed to the conception, writing, and editing of this manuscript

In individuals with an impaired methylation cycle this can provoke methylation issues such as high homocysteine, developmental disorders, gallbladder dysfunction, hormone imbalances, excess inflammation, poor growth and to name but a few

The K m values for the substrate 1-chloro-2,4-dinitrobenzene (CDNB) were 2.706 mM and 0.674 mM and were 6.146 mM and 3.559 mM for the substrate glutathione (GSH) for SjGST20 and SjGST22, respectively

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