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This AA-PE intermediate is further oxidized by ALOX enzymes, resulting in the generation of lipid hydroperoxides and the eventual induction of ferroptosis [96]

To determine if the non-functional mutants were expression or trafficking deficient, we analyzed the surface expression of all the mutants by biotinylation assay

To facilitate cellular uptake of FOXO4-DRI, it was designed as a fusion with HIV-TAT, a basic and hydrophilic sequence which allows energy-independent cellular uptake of cargo through transient pore formation (Herce and Garcia, 2007)
