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Briefly, cells were lysed at 4 C in a homogenization buffer (210 mM mannitol, 70 mM sucrose, 5 mM HEPES, pH 7.4) supplemented with EGTA (Sigma-Aldrich), albumin 0.5% and protease and phosphatase inhibitors (Sigma-Aldrich) and centrifuged at 600 g for 3 min, 4 C to pellet nuclei and heavy particles
The micrographs show uptake of GSH-targeted liposomes (red) by BCECs cultures (nuclei counterstained in blue) after incubation times of 1 A hr (A) and 2 hr (B)
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