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The beads were washed three times with low-salt washing buffer (150 mM KCl, 50 mM HEPES, pH 7.5, 0.05% (w/v) NP-40, 0.5 mM DTT and 1 protease inhibitor (Roche)), followed by RNase T1 treatment and a second incubation at 22 C for 10 min
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