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Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark

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Indeed, in spite of the fact that liposomal epitome makes items more steady, it increases production costs, makes cold-chain logistics more troublesome, and limits their accessibility in the market

An MTT cell viability assay demonstrated similar results and showed that mitochondrial viability was also significantly preserved in Immunocal-pretreated cells, compared to CGNs treated with SNP alone (Figure 4(c))

Unfortunately, they are being offered in unregulated, unsterile environments without proper clinical oversight