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Description
Lysate was applied on the column and contaminants were removed using Wash buffer 1 (50 mM Tris, pH 7.5, 150 mM NaCl, 20 mM imidazole and 2 mM TCEP) and Wash buffer 2 (50 mM Tris, pH 7.5, 1 M NaCl, 20 mM imidazole and 2 mM TCEP)

Final notes: You may draw up all of your injections at one time, or you may choose to only draw up your injection, one at a time, each day when you are taking your normal daily dose

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The convenience of a single injection appeals to many patients

It is also banned by WADA and USADA for all competitive athletes