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During Phase I many free radicals are generated making antioxidants a critical component to a well-rounded program
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Then antigen retrieval was performed with 1X Target Retrieval Solution, pH 6.0 (Dako) at 125 C for 4 min in a Retriever 2100 (Aptum Bio), followed with blocking using 10% goat serum in PBS for 60 min at RT
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