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PLoS ONE 8 , e59879 (2013)

MSH release-inhibiting factor-1 (MIF-1) is a tripeptide from the hypothalamus that controls the pituitary secretion of MSH

The liver is homogenized in ice-cold physiological saline and centrifuged

P53 activity maintains the functional integrity of mitochondrial morphology and structure, and mutations or deficiencies in p53 lead to significant reductions in cytochrome c oxidase (COX) activity and respiratory metabolism (198-200)
