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10.1016/j.cell.2008.07.021 Cell

Additional advantages of the GEM-handle are the combination with simple site-specific labeling chemistry guaranteeing consistent label positioning and the interchangeability of the fluorophore, which stands in contrast to trivalent platforms that necessitate comprehensive redesign and synthesis for fluorophore substitution

Concordant with the hypothesis that biguanides activate SKN-1 metabolic/innate immune and not oxidative stress defenses, phenformin treatment reduces expression of the canonical oxidative stress response gene gst-4 irrespective of bacterial diet source (Figure 6figure supplement 1AB), while reciprocally inducing expression of the innate immune response gene dod-24 in a manner dependent both upon skn-1 and ether lipids (Figure 6F and Figure 6figure supplement 1C)

It also stimulates Nrf2 signaling, which induces endogenous antioxidant enzyme expression after TBI

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