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(2018) 315:L545L52

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The relative fold difference in mRNA between samples was calculated by comparing the threshold cycle (Ct) at which product initially appeared above background according to 2 ( C t ) , where Ct is the difference between the control group and treatment group

Samples were prepared in the presence of (i) 1 mM phosphate buffer (pH 7.5), (ii) 1 mM phosphate buffer (pH 7.5) with 5% TFA (v/v), and (iii) 1 mM phosphate buffer (pH 7.5) with 1 mM TCEP