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Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

Start at 0.1-0.25 mg , not 0.5 mg

In conclusion, we demonstrate that exogenous GLP-2, resulting in supraphysiological plasma concentrations, dose-dependently inhibits postprandial gallbladder emptying in healthy men

This presents clinically as poor stamina, fatigue, difficultly focusing, changes in cardiovascular health and more