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For ECAR measurements, Glucose (10 mM), Oligomycin (1 M), and 2-Deoxyglucose (2-DG, glycolytic inhibitor

Each circle in (B) represent s an independent experiment

The raw sequencing data for this study can be found on the National Center for Biotechnology Informations Sequence Read Archive (SRA) website with the accession numbers SAMN27968563 to SAMN27968574

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Binding at a threefold excess molar concentration of zinc revealed the second binding site of the metal at His50 in the N terminus but no additional affinity was noted for the C terminus
