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Through SA--gal staining, we found that FOXO4-DRI also reduced the number of positive cells in the aorta of D-galactose induced aged mice (Figure 2C)

Cys-S n SH formed in this reaction either in the free form or incorporated in protein structure is an example of organic polysulfides

We also created a 5-oxoprolinase deletion ( oxp1 ) in this background

The pellet was resuspended in 2 ml ammonium chloride/Tris-chloride (pH 7.2) (erythrocyte lysing buffer), incubated at room temperature for 5 min, then supplemented with 1 ml FCS and centrifuged subsequently at 1433 r.p.m