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[ 125 I]GLP-1(7-36)NH 2 or [ 125 I]GIP(1-42)OH (2200 Ci/mmol, 95 % purity, final assay concentrations ~0.120.20 nM, PerkinElmer) in assay buffer (2.5 mM MgCl 2 [MilliporeSigma], 1.0 mM CaCl 2 [MilliporeSigma], 0.1% w/v fraction V fatty acid free bovine serum albumin [82-002-4, MilliporeSigma], 0.1% w/v bacitracin [11805, Affymetrix] in 25 mM HEPES [Thermo Fisher Scientific], pH 7.4, final concentrations) was added to peptide in assay buffer (concentration-response curves in DMSO, 3-fold acoustic direct dilution [Echo555, Labcyte], final DMSO concentration in the assay was 0.96%)

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The condition, called ischemic optic neuropathy (ION), occurs when blood flow to the optic nerve is reduced or blocked

The significant weight-loss effectiveness of the MK-801-GLP-1 conjugate, as well as the potent hypothalamic alterations in proteins and transcripts associated with NMDA receptor-related neuroplasticity, indicate that the changes in the compounds biodistribution driven by glucagon-like peptide-1-regulated targeting might effectively bypass MK-801 delivery to vagal afferents and targeted neuronal cells within the nucleus of tractus solitarius (NTS)