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The FFA-induced rise in intracellular Ca 2+ is substantially reduced when cells are cultured in a Ca 2+ free medium, and is abolished upon treatment of cells with the Ca 2+ channel inhibitor nicardipine [292] or when using BSA (which binds fatty acids) [251]

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However, aldosterone secretion is regulated by several factors besides ANG II, including potassium concentration, ACTH, and membrane depolarization of zona glomerulosa cells (93)