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CD-1 mice, Xenotech, catalog M1000) were diluted to 0.56 mg/mL in 100 mM PBS as a working solution

Characterized by cell injury and inflammatory cell infiltration, NASH is regarded as a more aggressive form of NAFLD possibly progressing to cirrhosis and hepatocellular carcinoma with limited treatment options (118) and with a history of NASH, the possibility of suffering liver and cardiovascular diseases is greatly increased (119)

Edholm T, Degerblad M, Grybck P, et al

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Gradually press down the plunger until it reaches the bottom and then withdraw the needle gently