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doi: 10.3389/fnut.2024.1398059

Afterward, the cells were washed with internalization buffer (HBBS supplemented with 1 mM CaCl 2 , 2.5 mM MgCl 2 , 20 mM HEPES, and 0.1% Pluronic F-68, pH 7.4) followed by addition of 100 M preheated fluorescein-O-acetic acid (acceptor, Sigma-Aldrich)

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