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The fold changes in gene expression were calculated with -actin as an internal control and using the ddCt method
Nat Med 8:738742 Mller TD, Finan B, Clemmensen C et al (2017) The new biology and pharmacology of Glucagon

The class encompasses multiple drugs varying in molecular structure, dosing frequencies, administration routes, and market prices

This study has some limitations that must be acknowledged

doi: 10.1016/j.mib.2024.102473
