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Competition binding with human GLP-1(7-36)NH 2 , GIP(1-42), tirzepatide, and semaglutide was performed essentially as described for homologous competition except that the assay buffer was 1.0 mM MgCl 2 , 2.5 mM CaCl 2 , 0.003% w/v Tween-20, 0.1% w/v bacitracin in 25 mM HEPES, pH 7.4, final concentrations with one Complete EDTA free protease inhibitor tablet added per 50 mL of buffer

Proc Natl Acad Sci U S A

Similar results were found in a clinical study in patients with T2DM, where administration of liraglutide, but not metformin, increased the relative abundance of Akkermansia , which is often associated with the improvement of gastric mucosa and also becomes severely depleted in diabetic patients [115]